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 The horizontal axis represents the H3 sequence and the vertical axis represents the amino acids by which the corresponding row is mutated.
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 For further validation, we selected histone H3 lysine 27 acetylation as a result of this modification has not been beforehand proven to be altered in CRC 19Protein immunostaining on western blots confirmed the increased K27 acetylation at H3 (Figure 3 ) and immunohistochemical staining of paired CRC and corresponding regular tissue sections revealed nuclear localisation of acetylated H3 protein (Figure four ).
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